5/20/2023 0 Comments K. v. rao moxtracultivars PR 106 and Pusa Basmati 1 (PB 1). oryza (Xoo) and belonging to nine wild Oryza species representing three genomic groups: AA (O.nivara, O.barthii, O.longistaminata, O.rufipogon and O.meridionalis), BBCC (O.punctata, O.malampuzhaensis and O.minuta) and CCDD (O.latifolia) were used to produce hybrids with two O.sativa (2n=24, AA). ![]() View full-textįourteen rice accessions resistant to bacterial leaf blight (BB), caused by Xanthomonasoryzae pv. Three DH lines, CR5-10, CR5-49, CR5-61 from CRHR5, and four DH lines, CR7-5, CR7-7, CR7-12 and CR7-52 from CRHR7, showed significant grain yield and quality characteristics and have been recommended for multi-location trials for subsequent release as new indica doubled haploid rice varieties. Physico-chemical characterization and cooking quality analyses revealed significant and acceptable values for grain length and width, alkali spreading value, amylose content and water uptake ratio of the selected DH lines. The yield levels of the DH lines ranged from 5 097–6 965kg/hm2 for CRHR5 and 5 141–7 235kg/hm2 for CRHR7, which were at par or higher than the parental hybrids. Nineteen promising DH lines of each hybrid were advanced to A2 generation for yield evaluation. Morpho-agronomic evaluation revealed 100% uniformity and stability for all the characters in the DH lines of both hybrids. Flow cytometric and pollen fertility analyses confirmed the DH ploidy status of the regenerations. ![]() of the rice anthers resulted in 243 and 186 fertile DH lines of CRHR5 and CRHR7, respectively. To alleviate the problem, we initiated a doubled haploid (DH) breeding approach to develop new lines from two elite indica rice hybrids (CRHR5 and CRHR7) through rapid fixation of homozygosity in the recombinants. ![]() Despite significant yield advantage over inbred rice, the adoption rate of hybrid rice in India is very low due to the high seed cost and poor quality of the produce.
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